Precision Run-On (PRO) Sequencing Bowtie2 alignment
The PRO-seq experiment was done on CHM13 cells and sequenced for 75bp single-ended reads.
Reads were trimmed and filtered and then aligned to the T2T CHM13 v1.0 assembly (released on 20200922) using bowtie2/2.3.5.1 with the following parameters:
-N 0
-k 100
Savannah Hoyt
Rachel O'Neill