Description

Precision Run-On (PRO) Sequencing Bowtie2 alignment anchored to single-copy 21-mers found in assembly-filtered PCR-free WGS reads

Methods

The PRO-seq experiment was done on CHM13 cells and sequenced for 75bp single-ended reads.

Reads were trimmed and filtered and then aligned to the T2T CHM13 v1.0 assembly (released on 20200922) using bowtie2/2.3.5.1 with the following parameters:

-N 0

-k 100

Following alignment, the reads were anchored to single-copy 21-mers found in assembly-filtered PCR-free WGS reads generated with meryl/1.2 using GenomeBrowser/20180626 overlapSelect with the following parameters:

-overlapBases=21

Display Conventions and Configuration

Data access

Release history

1. 2020-09-22: CHM13 v1.0 release (chm13.draft_v1.0.fasta)

Contacts

• Rachel O'Neill <rachel.oneill@uconn.edu>
• Savannah Hoyt <savannah.klein@uconn.edu>

Credits

Rachel O'Neill

Savannah Hoyt

Arang Rhie (Meryl software)