CHM13 PRO-seq Bowtie2 alignments and Meryl unique k-mer filtering (split strands)
The PRO-seq experiment was done on CHM13 cells and sequenced for 75bp single-ended reads.
Reads were trimmed and filtered before mapped with Bowtie2 -N 0 -k 100
Mapped reads were then filtered through unique genome-wide 51mers
Savannah Hoyt (PROseq library prep & sequencing; all mapping & filtering)
Rachel O'Neill
Arang Rhie (Meryl software)