HG002 Cut and Run low salt only BWA-F3852 alignments and Meryl unique k-mer filtering from the DEC 2020 and FEB 2021 runs
The Cut and Run experiments were done on HG002 cells and sequenced for 150bp paired-end reads.
Reads were trimmed and filtered (q20) before mapped to chm13_HG002X_HG38Y.fasta with bwa mem -k 50 -c 1000000 and samtools filtered (F3852)
losalt mapped reads were then filtered through unique 51mers (including chm13 (minus chrX) + hg002 (chrX only) + no chrY)
Gina Caldas & Nick Altemose (Cut & Run experiments)
Karen Miga (mapping and Samtools filtering)
Savannah Hoyt; Rachel O'Neill (k-mer filtering)
Arang Rhie (Meryl software; unique 51mers)