Description

CHM13 PRO-seq Bowtie2 (-k 100) alignments to CHM13v1.1 and Meryl unique k-mer filtering (stranded & not stranded)

Methods

The PRO-seq experiment was done on CHM13 cells and sequenced for 75bp single-ended reads.

Reads were adapter trimmed, quality filtered and D. melanogaster spike-ins were removed.

Reads were mapped with Bowtie2 -k 100 and then filtered through unique genome-wide 21 or 51mers

Display Conventions and Configuration

Data access

Release history

1. CHM13v1.1 assembly (04.27.21)

Contacts

• Savannah Hoyt <savannah.klein@uconn.edu>

Credits

Savannah Hoyt (PROseq library prep & sequencing; all mapping & filtering)

Rachel O'Neill

Arang Rhie (Meryl software for generation of unique kmers)