Description

Precision Run-On (PRO) Sequencing alignment)

Methods

The PRO-seq experiment was done on CHM13 cells and sequenced for 75bp single-ended reads.

Reads were trimmed and filtered and then aligned to the T2T CHM13 assembly (20200727) using STAR/2.7.1a with the following parameters:

--outFilterMultimapNmax 100

--outFilterMismatchNmax 0

Display Conventions and Configuration

Data access

Data is available on

• globus:/team-epigenetics/20200727_PROseq_CHM13/CHM13-5A_proseq_cutadapt-2_bt2-vs_filtered_STAR-100mm-0m-chm13-v1.0_Aligned.sortedByCoord_POS.bedgraph
• globus:/team-epigenetics/20200727_PROseq_CHM13/CHM13-5A_proseq_cutadapt-2_bt2-vs_filtered_STAR-100mm-0m-chm13-v1.0_Aligned.sortedByCoord_NEG.bedgraph

Release history

1. 2020-09-04: initial t2tChm13_20200727 release

Contacts

• Rachel O'Neill <rachel.oneill@uconn.edu>
• Savannah Hoyt <savannah.klein@uconn.edu>

Credits

Rachel O'Neill

Savannah Hoyt