CHM13 PRO-seq and RNA-seq Bowtie2-F3852 alignments and Meryl unique k-mer filtering
The PRO-seq experiment was done on CHM13 cells and sequenced for 75bp single-ended reads.
The native RNA-seq libraries were done on CHM13 cells using oligodT during library prep and sequenced for 150bp paired-end reads.
Reads were trimmed and filtered before mapped with Bowtie2 -N 0 -k 100 and samtools filtered (F3852)
Mapped reads were then filtered through two different sets of genome-wide unique k-mers: 21mers, 51mers
Savannah Hoyt (PROseq library prep & sequencing; all mapping & filtering)
Rachel O'Neill
Megan Dennis (RNAseq library prep & sequencing)
Colin Shew (RNAseq library prep & sequencing)
Arang Rhie (Meryl software)