Description

CHM13 RNA-seq Bowtie2 k100 alignments and Meryl unique k-mer filtering (split strands)

Methods

The native RNA-seq libraries were done on CHM13 cells using oligodT during library prep and sequenced for 150bp paired-end reads.

Reads were trimmed and filtered before mapped with Bowtie2 -k 100 and then samtools F1548 filtered for paired-reads only

Mapped reads were then filtered through unique genome-wide 21mers & 51mers

Display Conventions and Configuration

Data access

Release history

1. 2020-09-22: CHM13 v1.0 release (chm13.draft_v1.0.fasta)

Contacts

• Savannah Hoyt <savannah.klein@uconn.edu>

Credits

Megan Dennis (RNAseq library prep & sequencing)

Colin Shew (RNAseq library prep & sequencing)

Savannah Hoyt (mapping & filtering)

Rachel O'Neill

Arang Rhie (Meryl software)